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KMID : 0380620110430050633
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Korean Journal of Food Science and Technology
2011 Volume.43 No. 5 p.633 ~ p.640
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Active Polysaccharide and Immune Enhancement of Ganoderma lucidum Mycelium Cultured in Mushroom Complete Medium Supplemented with Ginseng Extract
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Kim Hoon
Jeong Jae-Hyun
Jeong Heon-Sang
Hwang Jong-Hyun
Yu Kwang-Won
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Abstract
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After Ganoderma lucidum was cultured in mushroom complete medium (MCM) supplemented with ginseng extract (GE), crude polysaccharide (GL-GE-CP) was fractionated from mycelium. Among GL-GE-CP from mycelium in MCM supplemented with 5, 10, and 15% GE (v/v ratio of MCM to GE), GL-GE- 15-CP (15% GE) most significantly enhanced macrophage stimulation and intestinal immune system modulating activity compared with GL-CP in MCM without GE. When GL-GE-15-CP was further fractionated on DEAE-Sepharose CL-6B, GL-GE-15-CP-II displayed more potent activity than subfractions from GL-CP on macrophage stimulation, interleukin-12 production, and intestinal immune system modulation (1.75-, 5.68-, and 1.76-fold, respectively). Anti-metastasis effect against colon 26-M3.1 carcinoma cells was also enhanced by GL-GE-15-CP-II (72.8% inhibition). In addition, GL-GE- 15-CP-II contained neutral sugar (83.00%) and uronic acid (9.11%), and consisted of Ara, Man, Gal and Glc (molar ratio of 0.39:0.50:0.75:1.00). Furthermore, GE supplementation helped to enhance the immunomodulation in G. lucidum, and it is assumed that neutral polysaccharides play an important role.
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KEYWORD
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Ganoderma lucidum mycelium, ginseng extract, mushroom complete medium, polysaccharide, immunomodulation
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